產品(pǐn)中心(xīn)
標(biāo)記一抗
英(yīng)文(wén)名稱Anti-phospho-ATF2 (Thr53)/FITC
中文名稱:FITC標(biāo)記的磷酸(suān)化(huà)活化(huà)復製(zhì)因子2抗(kàng)體
別(bié) 名(míng)ATF2(Phospho-Thr53); ATF2(Phospho-T53); p-ATF2(Thr53); p-ATF2(T53); CREB 2; HB 16; Activating Transcription Factor 2; ATF 2; Atf-2; ATF2 protein; cAMP Response Element Binding Protein 2; cAMP response element binding protein CRE BP1; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; CRE BP1; CRE-BP; CREB 2; CREB2; CREBP1; Cyclic AMP dependent transcription factor ATF 2; Cyclic AMP-responsive; ATF2_HUMAN.
規格(gé):100ul
說(shuō) 明 書100ul
產(chǎn)品類(lèi)型(xíng)磷(lín)酸化抗(kàng)體
研究領域腫瘤 細(xì)胞(bāo)生物(wù) 免疫學 信(xìn)號轉(zhuǎn)導 細(xì)胞凋(diāo)亡 轉錄調節因子
抗體(tǐ)來(lái)源Rabbit
克隆類(lèi)型(xíng)Polyclonal
交(jiāo)叉(chā)反應 Human, Mouse, Rat, Cow, Horse, Rabbit,
產(chǎn)品(pǐn)應用(yòng)IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量54kDa
性 狀Lyophilized or Liquid
濃(nóng) 度(dù)1mg/ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human ATF2 around the phosphorylation site of Thr53
亞 型(xíng)IgG
純化(huà)方法affinity purified by Protein A
儲 存 液(yè)0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存(cún)條(tiáo)件(jiàn)Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品(pǐn)介紹background:
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012].
Function:
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Subunit:
Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Interacts with SMAD3 and SMAD4. Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity.
Subcellular Location:
Nucleus.
Tissue Specificity:
Abundant expression seen in the brain.
Post-translational modifications:
Phosphorylation of Thr-69 by MAPK14 and MAPK11, and at Thr-71 by MAPK1/ERK2, MAPK3/ERK1, MAPK11, MAPK12 and MAPK14 in response to external stimulus like insulin causes increased transcriptional activity. Phosphorylated by PLK3 following hyperosmotic stress. Also phosphorylated and activated by JNK and CaMK4.
中文名稱:FITC標(biāo)記的磷酸(suān)化(huà)活化(huà)復製(zhì)因子2抗(kàng)體
別(bié) 名(míng)ATF2(Phospho-Thr53); ATF2(Phospho-T53); p-ATF2(Thr53); p-ATF2(T53); CREB 2; HB 16; Activating Transcription Factor 2; ATF 2; Atf-2; ATF2 protein; cAMP Response Element Binding Protein 2; cAMP response element binding protein CRE BP1; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; CRE BP1; CRE-BP; CREB 2; CREB2; CREBP1; Cyclic AMP dependent transcription factor ATF 2; Cyclic AMP-responsive; ATF2_HUMAN.
詳細介紹(shào)：
規格(gé):100ul
說(shuō) 明 書100ul
產(chǎn)品類(lèi)型(xíng)磷(lín)酸化抗(kàng)體
研究領域腫瘤 細(xì)胞(bāo)生物(wù) 免疫學 信(xìn)號轉(zhuǎn)導 細(xì)胞凋(diāo)亡 轉錄調節因子
抗體(tǐ)來(lái)源Rabbit
克隆類(lèi)型(xíng)Polyclonal
交(jiāo)叉(chā)反應 Human, Mouse, Rat, Cow, Horse, Rabbit,
產(chǎn)品(pǐn)應用(yòng)IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量54kDa
性 狀Lyophilized or Liquid
濃(nóng) 度(dù)1mg/ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human ATF2 around the phosphorylation site of Thr53
亞 型(xíng)IgG
純化(huà)方法affinity purified by Protein A
儲 存 液(yè)0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存(cún)條(tiáo)件(jiàn)Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
相關資(zī)料：
產品(pǐn)介紹background:
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012].
Function:
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Subunit:
Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Interacts with SMAD3 and SMAD4. Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity.
Subcellular Location:
Nucleus.
Tissue Specificity:
Abundant expression seen in the brain.
Post-translational modifications:
Phosphorylation of Thr-69 by MAPK14 and MAPK11, and at Thr-71 by MAPK1/ERK2, MAPK3/ERK1, MAPK11, MAPK12 and MAPK14 in response to external stimulus like insulin causes increased transcriptional activity. Phosphorylated by PLK3 following hyperosmotic stress. Also phosphorylated and activated by JNK and CaMK4.
