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英文名稱 Anti-phospho-Bid (Ser78)
中(zhōng)文名稱： 磷(lín)酸(suān)化(huà)BH3結(jié)構(gòu)域凋亡(wáng)誘(yòu)導蛋(dàn)白(bái)抗(kàng)體
別 名(míng) Bid (phospho S78); p-Bid (phospho S78); BH3 interacting domain death agonist; p22 BID; BID;) BH3-interacting domain death agonist; AltName: BH3-interacting domain death agonist p15; BH3-interacting domain death agonist p13; p13 BID; BH3-interacting domain death agonist p11; p11 BID; BID_MOUSE.
濃(nóng) 度 1mg/1ml
規 格 0.1ml/100μg
抗(kàng)體(tǐ)來源(yuán) Rabbit
克(kè)隆(lóng)類型 polyclonal
交(jiāo)叉(chā)反(fǎn)應 Mouse, Rat
產(chǎn)品類(lèi)型(xíng) 一抗 磷(lín)酸(suān)化抗體
研(yán)究領(lǐng)域(yù) 腫(zhǒng)瘤 細胞(bāo)生(shēng)物(wù) 信號轉(zhuǎn)導 細(xì)胞凋亡
蛋白分子(zǐ)量 predicted molecular weight: 22kDa
性 狀 Lyophilized or Liquid
免 疫(yì) 原(yuán) KLH conjugated Synthesised phosphopeptide derived from mouse Bid around the phosphorylation site of Ser78
亞 型(xíng) IgG
純化方法(fǎ) affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產品(pǐn)應(yīng)用(yòng) WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蠟(là)切(qiē)片需(xū)做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件(jiàn) Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
Function : The major proteolytic product p15 BID allows the release of cytochrome c. Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
Subunit : Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2.
Subcellular Location : Cytoplasm (By similarity). Mitochondrion membrane. Note=When uncleaved, it is predominantly cytoplasmic. BH3-interacting domain death agonist p15: Mitochondrion membrane. Note=Translocates to mitochondria as an integral membrane protein. BH3-interacting domain death agonist p13: Mitochondrion membrane. Note=Associated with the mitochondrial membran. Isoform 1: Cytoplasm. Isoform 3: Cytoplasm. Isoform 2: Mitochondrion membrane. Note=A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively.
Tissue Specificity : Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
Post-translational modifications : TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Database links : UniProtKB/Swiss-Prot: P70444.2
中(zhōng)文名稱： 磷(lín)酸(suān)化(huà)BH3結(jié)構(gòu)域凋亡(wáng)誘(yòu)導蛋(dàn)白(bái)抗(kàng)體
別 名(míng) Bid (phospho S78); p-Bid (phospho S78); BH3 interacting domain death agonist; p22 BID; BID;) BH3-interacting domain death agonist; AltName: BH3-interacting domain death agonist p15; BH3-interacting domain death agonist p13; p13 BID; BH3-interacting domain death agonist p11; p11 BID; BID_MOUSE.
詳(xiáng)細(xì)介紹：
濃(nóng) 度 1mg/1ml
規 格 0.1ml/100μg
抗(kàng)體(tǐ)來源(yuán) Rabbit
克(kè)隆(lóng)類型 polyclonal
交(jiāo)叉(chā)反(fǎn)應 Mouse, Rat
產(chǎn)品類(lèi)型(xíng) 一抗 磷(lín)酸(suān)化抗體
研(yán)究領(lǐng)域(yù) 腫(zhǒng)瘤 細胞(bāo)生(shēng)物(wù) 信號轉(zhuǎn)導 細(xì)胞凋亡
蛋白分子(zǐ)量 predicted molecular weight: 22kDa
性 狀 Lyophilized or Liquid
免 疫(yì) 原(yuán) KLH conjugated Synthesised phosphopeptide derived from mouse Bid around the phosphorylation site of Ser78
亞 型(xíng) IgG
純化方法(fǎ) affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產品(pǐn)應(yīng)用(yòng) WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蠟(là)切(qiē)片需(xū)做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件(jiàn) Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
相關資料：
產(chǎn)品介紹 Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
Function : The major proteolytic product p15 BID allows the release of cytochrome c. Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
Subunit : Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2.
Subcellular Location : Cytoplasm (By similarity). Mitochondrion membrane. Note=When uncleaved, it is predominantly cytoplasmic. BH3-interacting domain death agonist p15: Mitochondrion membrane. Note=Translocates to mitochondria as an integral membrane protein. BH3-interacting domain death agonist p13: Mitochondrion membrane. Note=Associated with the mitochondrial membran. Isoform 1: Cytoplasm. Isoform 3: Cytoplasm. Isoform 2: Mitochondrion membrane. Note=A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively.
Tissue Specificity : Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
Post-translational modifications : TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Database links : UniProtKB/Swiss-Prot: P70444.2
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