產(chǎn)品中(zhōng)心(xīn)
一抗(kàng)
英文名(míng)稱(chēng) Anti-BNIP3/NIP3
中文(wén)名稱： 促凋亡調節(jié)蛋白BNIP3抗體(tǐ)
別 名(míng) BCL2 Adenovirus E1B 19kDa Interacting Protein 3; BCL2/adenovirus E1B 19 kDa protein interacting protein 3; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BNIP 3; BNIP3; BNIP3_HUMAN; NIP 3; NIP3.
濃(nóng) 度(dù) 1mg/1ml
規 格 0.1ml/100μg 0.2ml/200μg
抗體來(lái)源(yuán) Rabbit
克(kè)隆類(lèi)型(xíng) polyclonal
交叉(chā)反(fǎn)應 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse
產(chǎn)品(pǐn)類型 一(yī)抗
研(yán)究(jiū)領域(yù) 腫瘤(liú) 心(xīn)血管 免(miǎn)疫(yì)學 細胞凋亡(wáng)
蛋白分(fēn)子量(liáng) predicted molecular weight: 22kDa
性 狀(zhuàng) Lyophilized or Liquid
免(miǎn) 疫 原(yuán) KLH conjugated synthetic peptide derived from human BNIP3
亞 型 IgG
純(chún)化(huà)方(fāng)法(fǎ) affinity purified by Protein A
儲 存(cún) 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
產(chǎn)品(pǐn)應用(yòng) WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片(piàn)需(xū)做抗(kàng)原(yuán)修(xiū)復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保(bǎo)存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產品介紹 Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.
Function : Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway.
Subunit : Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1. Interacts (via BH3 domain) with SPATA18 (via coiled-coil domains).
Subcellular Location : Mitochondrion. Mitochondrion membrane. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria.
Similarity : Belongs to the NIP3 family.
BNIP3蛋白在缺氧條件下(xià)可(kě)誘(yòu)導(dǎo)細胞發生細胞凋(diāo)亡。BNIP3促細(xì)胞凋(diāo)亡綫粒體途(tú)徑(jìng)根(gēn)據有無caspase的參(cān)與(yǔ)而具有不同的(dí)作用機製(zhì)，也是研究(jiū)的(dí)熱點(diǎn)。BNIP3還可誘(yòu)導細(xì)胞自(zì)噬(shì)。BNIP3全稱(chēng)為(wéi)Bcl2/腺(xiàn)病毒(dú)E1B19kD相(xiāng)互作用蛋白(bái)3。(Bcl2/adenovirusE1B19kDainteractingprotein3，BNIP3)，是(shì)新近(jìn)發現的BH3only亞家(jiā)族成(chéng)員之一(yī)，也是低氧(yǎng)敏感(gǎn)的(dí)凋亡基因。在(zài)化學性(xìng)低氧(yǎng)誘導細(xì)胞(bāo)凋(diāo)亡的(dí)過程(chéng)中，ATP是(shì)BNIP3導致細胞(bāo)凋亡(wáng)的關(guān)鍵性因素之(zhī)一(yī)。單獨ATP下降不誘(yòu)導(dǎo)細胞凋亡，但ATP下降到(dào)一(yī)定程(chéng)度(dù)能(néng)促(cù)進BNIP3向(xiàng)綫粒體轉位(wèi)，從(cóng)而(ér)誘導(dǎo)了BNIP3相關(guān)的凋(diāo)亡。這可能是(shì)低(dī)氧誘(yòu)導(dǎo)BNIP3表達導(dǎo)致細(xì)胞(bāo)凋(diāo)亡的(dí)重要機製之一(yī)。
中文(wén)名稱： 促凋亡調節(jié)蛋白BNIP3抗體(tǐ)
別 名(míng) BCL2 Adenovirus E1B 19kDa Interacting Protein 3; BCL2/adenovirus E1B 19 kDa protein interacting protein 3; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BNIP 3; BNIP3; BNIP3_HUMAN; NIP 3; NIP3.
詳細介(jiè)紹：
濃(nóng) 度(dù) 1mg/1ml
規 格 0.1ml/100μg 0.2ml/200μg
抗體來(lái)源(yuán) Rabbit
克(kè)隆類(lèi)型(xíng) polyclonal
交叉(chā)反(fǎn)應 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse
產(chǎn)品(pǐn)類型 一(yī)抗
研(yán)究(jiū)領域(yù) 腫瘤(liú) 心(xīn)血管 免(miǎn)疫(yì)學 細胞凋亡(wáng)
蛋白分(fēn)子量(liáng) predicted molecular weight: 22kDa
性 狀(zhuàng) Lyophilized or Liquid
免(miǎn) 疫 原(yuán) KLH conjugated synthetic peptide derived from human BNIP3
亞 型 IgG
純(chún)化(huà)方(fāng)法(fǎ) affinity purified by Protein A
儲 存(cún) 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
產(chǎn)品(pǐn)應用(yòng) WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片(piàn)需(xū)做抗(kàng)原(yuán)修(xiū)復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保(bǎo)存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
相(xiāng)關資(zī)料：
產品介紹 Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.
Function : Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway.
Subunit : Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1. Interacts (via BH3 domain) with SPATA18 (via coiled-coil domains).
Subcellular Location : Mitochondrion. Mitochondrion membrane. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria.
Similarity : Belongs to the NIP3 family.
BNIP3蛋白在缺氧條件下(xià)可(kě)誘(yòu)導(dǎo)細胞發生細胞凋(diāo)亡。BNIP3促細(xì)胞凋(diāo)亡綫粒體途(tú)徑(jìng)根(gēn)據有無caspase的參(cān)與(yǔ)而具有不同的(dí)作用機製(zhì)，也是研究(jiū)的(dí)熱點(diǎn)。BNIP3還可誘(yòu)導細(xì)胞自(zì)噬(shì)。BNIP3全稱(chēng)為(wéi)Bcl2/腺(xiàn)病毒(dú)E1B19kD相(xiāng)互作用蛋白(bái)3。(Bcl2/adenovirusE1B19kDainteractingprotein3，BNIP3)，是(shì)新近(jìn)發現的BH3only亞家(jiā)族成(chéng)員之一(yī)，也是低氧(yǎng)敏感(gǎn)的(dí)凋亡基因。在(zài)化學性(xìng)低氧(yǎng)誘導細(xì)胞(bāo)凋(diāo)亡的(dí)過程(chéng)中，ATP是(shì)BNIP3導致細胞(bāo)凋亡(wáng)的關(guān)鍵性因素之(zhī)一(yī)。單獨ATP下降不誘(yòu)導(dǎo)細胞凋亡，但ATP下降到(dào)一(yī)定程(chéng)度(dù)能(néng)促(cù)進BNIP3向(xiàng)綫粒體轉位(wèi)，從(cóng)而(ér)誘導(dǎo)了BNIP3相關(guān)的凋(diāo)亡。這可能是(shì)低(dī)氧誘(yòu)導(dǎo)BNIP3表達導(dǎo)致細(xì)胞(bāo)凋(diāo)亡的(dí)重要機製之一(yī)。
